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Arch. med. res ; 27(1): 67-70, 1996. tab, ilus
Article in English | LILACS | ID: lil-200293

ABSTRACT

The identification of human IgG immunoglobulins on the surface of Vibrio cholerae O1, and Haemophilus influenzae type b microorganisms was assessed via a flow cytometric technique. A group of 31 healthy mother-full term nerwborn duo sera from a non-endemic cholera area was assayed. The sera of mothers and full-term newborns against both microorganisms were compared. The mean fluorescent intensity of the samples was not different at the 0.05 significance level by paired t-test. On the other hand, the immunoglobulins of newborn and mothers for V. cholerae O1 was notably lower when compared with H. influenzae type b microorganisms (p<0.05 by paried t-test, t=-5.570 for mother' sera, and t=-7.496 for the sera of the newborns). These data provide circumstantial evidence that LFC techique would be useful on bacteria-related serology


Subject(s)
Humans , Female , Brucella melitensis/isolation & purification , Flow Cytometry/methods , Haemophilus influenzae/isolation & purification , Immunoglobulin G/immunology , Immunoglobulins/isolation & purification , Maternal and Child Health , Bacterial Outer Membrane Proteins/isolation & purification , Serologic Tests , Vibrio cholerae/isolation & purification
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